Fig. 11. mRNA expression of VEGF, FGF2 and Caspase 3 in both wild/control and TSP1 knockout (KO) luteal cells. CRISPR-Cas9 mediated gnomic editing technology was used to produce TSP1 knockout luteal cells. Cells from both the groups were cultured up to 75-80% confluency over a period of 72 h (n = 3/condition). Cells lysate was used for the extraction of total RNA and cDNA preparation. Two-way ANOVA with Tukey's HSD was done to analyse the mRNA expression of VEGF, FGF2 & Caspase. Each point in the line chart indicates Mean ± SEM. The different superscripts denote statistical significance (p< 0.05).